GLISONI Romina Julieta
congresos y reuniones científicas
Exosomes as nanocarriers of apoTransferrin for therapeutic use
MATTERA V.; GLISONI RJ.; CORREALE JD.; PASQUINI J.
Mar del Plata
Congreso; SAIC SAI SAFIS-NANOMED-ar 2018; 2018
Exosomes are biological extracelullar vesicles with a diameter ranging between 20 and 100 nm which are fundamental for intercellular communication, RNA, DNA and protein transport. Exosome capacity for biologic information transfer makes them an attractive tool for therapeutic agent nano-delivery to specific target cells. In addition to their small diameter, the exosome bilipidic structure protects the cargo from degradation and immunological detection; allows them to overpass the blood brain barrier and increases cargo molecule stability, solubility and bioavailability. Previous studies in our laboratory demonstrated the pro-myelinating and differentiating effect of apoTransferrin (aTf) in the central nervous system (CNS). The present work focuses on aTf nano-encapsulation for targeting to oligodendroglial cells in the CNS through intranasally administered endosomes in cuprizone- demyelinated mice.We isolated exosomes from different sources; human plasma, mouse plasma, neuroblastoma cell line (N2a), oligodendroglioma cell line (OLN-93) and primary culture of astrocytes. We characterized exosomes by Western blot using typical exosome markers CD63, alix and Tsg101 to verify their purity and identity. To verify their structural integrity and average size, we performed scanning electron microscopy (SEM) and dynamic light scattering (DLS). We conclude that the nanoparticles isolated are exosomes and that their structure is not compromised, allowing us to proceed to the loading, tracking and targeting phase of this study.