GENOMICS AND PROTEOMICS OF BACTERIOCIN-PRODUCING STRAIN Pseudomonas fluorescens SF4C

AGUSTINA GODINO; MARICRUZ FERNANDEZ; ANALÍA PRÍNCIPE; SONIA FISCHER

Córdoba

Congreso; LII Reunión Anual-Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Bacteriocins are narrow-spectrum antimicrobial with high potential for application as biocontrol agents. Characterization of new bacteriocins with different action spectra becomes increasingly relevant. In this context, we perform a genome mining study to predict bacteriocin genes in the draft genome of P. fluorescensSF4c. This in silico analysis revealed the presence of a complete tailocin (phage-like bacteriocins) cluster with structural genes for R (contractile) and F (flexible) -type tailocins. Moreover, two genes corresponding to S-type pyocins (soluble proteins) with their cognate immunity proteins were also identified in the SF4c genome. In order to analyze if the predicted bacteriocins are produced by SF4c a proteomics study was performed. Extracts of supernatants of SF4c cultures induced with mitomycin C were digested with trypsin and analyzed by nano-HPLC coupled to mass spectrometer with Orbitrap technology (Mass Spectrometry Service of CEQUIBIEM-UBA). The spectra were analyzed against SF4c proteome obtained from draft genome. Data analysis showed that all bacteriocin predicted from genome were identified in the SF4c extract, including the 27 structural proteins of R and F tailocins and the 2 pyocins S with their respective immunity proteins. These results show thatP. fluorescensSF4c is able to produce various bacteriocins with different structures and properties