INVESTIGADORES
CORBALAN Natalia Soledad
congresos y reuniones científicas
Título:
THE SBMA LOCUS IS INVOLVED IN VIRULENCEASSOSIATED PHENOTYPES
Autor/es:
CORBALÁN ,NATALIA S.; POMARES, MARÍA FERNANDA; LAMA MJ; DE CRISTÓBAL ,RICARDO E.; VINCENT ,PAULA A.
Reunión:
Congreso; 47 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2011
Resumen:
SbmA protein is involved in the transport of MccB17, MccJ25,
bleomycin and proline rich peptides into the Escherichia coli
cytoplasm. In Brucella abortus, Sinorhizobium meliloti and
Mycobacterium tuberculosis the SbmA homologue, BacA, is
essential for the chronic infection of these pathogens. We evaluated
SbmA role in the Salmonella Typhimurium ability to infect and
replicate within macrophages. The sbmA mutant strain showed
increased intracellular replication compared to parental strain.
Further assays demonstrated that this phenomenon was caused by
increased resistance to the acidic pH present in the macrophage
environment. Biofilm formation, aggregation and swarming are
important bacterial traits that affect pathogen chances of survival in
competitive environments and the ability to infect hosts. Assays
demonstrated that sbmA absence significantly enhanced biofilm,
aggregation and swarming phenotypes. Additionally, mutation in
yaiW, gene transcriptionally related to sbmA, affected biofilm and
swarming in the same way as sbmA mutation did. However, the
yaiW mutant showed a reduction in the aggregation phenotype. A
gene profile expression assay of sbmA and yaiW mutans showed
that the transcription levels of genes implicated in swarming and
motility were enhanced in those mutants. These results suggest that
sbmA is involved in important physiological roles related to
virulence.