ECs from peripheral blood mononuclear cells is modulated in an LPS-induced preterm murine model

MARVALDI, CAROLINA; SCHANDER, JULIETA AYLEN; AISEMBERG, JULIETA; FRANCHI, ANA MARIA; WOLFSON, MANUEL LUIS

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC, SAI, AAFE, NANOMED-AR

Preterm birth (PTB) is the leading cause of mortality and morbidity in neonates. The endocannabinoid system (ECs) is one of several signaling pathways involved in different aspects of the physiopathology of reproduction, comprising the enzyme that synthesizes AEA, (N-acylphosphatidylethanolamine-specific phospholipase D, NAPE-PLD), the enzyme that hydrolase AEA (fatty acid amide hydrolase, FAAH), and the receptors CB1, CB2 and TRPV1. Previous works from our lab have demonstrated that LPS altered FAAH activity in deciduas and PBMC in an embryo resorption model. Using a murine model of preterm labor, induced by two injections of LPS on day 15 of pregnancy, we demonstrated that ECs of the decidua is involved in the triggering of PTB. Due to the decidua is highly infiltrated by immune cells, in this study we investigated if the ECs of peripheral blood mononuclear cells (PBMC) is modulated in our LPS-induced preterm labor model.We observed that CB1 receptor protein levels increased in PBMC after LPS treatment (p