Possible involvement of prostaglandin E2 (PGE2) in bradykinin (BK) B1 receptor mediated contractile response in human umbilical vein (HUV).

DARAY, F.; SERRANO, S.; MINVIELLE, I.; WOLFSON, M.; NOVAK, W.; REY ARES, V; ROTHLIN, R.

Corrientes, Argentina.

Congreso; XXXIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental.; 2001

&amp;amp;amp;amp;amp;amp;lt;!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:21.0cm 842.0pt; margin:70.9pt 62.95pt 17.85pt 3.0cm; mso-header-margin:35.45pt; mso-footer-margin:35.45pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&amp;amp;amp;amp;amp;amp;gt; Introduction and goals: in HUV our group have demonstrated that the prostanoids, sintetized by the cyclo-oxygenase-2 are involved in the contractile response to des-Arg9-BK, BK B1 receptor agonist (Naunyn-Schmiedeberg ´s Arch Pharmacol. 364: 149-156 _,2001). The aim of this study was to evaluate whether the PGE2 is one of the prostanoid/s involved in this response and which is/are the EP receptors present in this tissue.  Methods and results: HUV rings were mounted under isometric Kreb´s solution at 37°C. After 5h, concentration-response curves (CRCS) were obtained to des-Arg9-BK. Exposure to  30ug of AH6809 (EP1 antagonist), produced a significant rightward shift of the CRC to des-Arg9-BK (pCE50 control: 7.50±0.09; AH:6.95±0.05, p<.O5). ln other rings CRCS to PGE2 were performed and CRCS were shifted to the right when 1 uM of AH6809 or SCl9220 (EP1 selective antagonist) were applied (pCE50 6.22±0.08; AH: 5.40±0.45; sc; 5.84 ±0.15, p<.05).  These results allowed us to estimate pKb values for AH: 6.74 and SC:6.14 which   are similar to the pA2 values published for EP1 receptor, AH: 6.8  and SC: 5.4. Conclusion: this results suggests that  PGE2 is involved in BK B1 receptor signaling robably  through EP1 receptor stimulus in HUV.