In vivo and in vitro propagation of macela: a medicinal-aromatic native plant with ornamental potential

GUARINIELLO, JULIAN; IANNICELLI, JESICA; PERALTA, PATRICIA; ESCANDON, ALEJANDRO SALVIO

Ornamental Horticulture

Sociedade Brasileira de Floricultura e Plantas Ornamentais

Año: 2018 vol. 24 p. 361 - 370

2447-536X

Achyrocline satureioides is a shrub native from South America. In popular medicine it is used in infusions such as digestive,carminative, antispasmodic, eupeptic and emmenagogue. However, its main use is as an ingredient in the liquor industry. Commercialexploitation is carried out through the collection of natural populations in an unsustainable way. The micropropagation of A.satureioides will allow its massive propagation and it will settle a base for its domestication. For this, a clone denominated as M1-5was first propagated by cuttings. Subsequently, nodal segments obtained from young stems were disinfected by a standard methodand cultured on MS medium. These shoots were used as a source of explants for subsequent assays. For its in vitro establishmentMS medium and WPM were tested. Once the culture was established, the responses of the explants to increasing concentrations of6-benzylaminopurine (BAP) (0.0; 0.5; 2.5 and 5.0 ìM) with and without 0.05 ìM á-naphthalene acetic acid (NAA) on WPM asbasal medium were studied during 35 days. The proliferation of buds, the presence of callus and the number and length of the rootswere evaluated. All of the ?macela? cuttings in vivo propagated rooted and developed satisfactorily under the conditions tested.The application of 5.0 ìM BAP alone generated the best multiplication rate, so it was selected as the multiplication medium. Denovo shoots rooted spontaneously and finally, transferred to the greenhouse. Here in it was possible to establish a micropropagationprotocol not only for the production of plantlets of selected clones but also for the application of biotechnological tools in thedevelopment of A. satureioides germplasm