Genetic Relationships among Strains of the Aspergillus niger

FERRACIN, LARA MUNIQUE; FRISVAD, JENS CHRISTIAN; TANIWAKI, MARTA HIROMI; IAMANAKA, BEATRIZ THIE; SARTORI, DANIELE; SCHAPOVALOFF, MARIA ELENA; FUNGARO, MARIA HELENA

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY

INST TECNOLOGIA PARANA

Año: 2009 vol. 52 p. 241 - 248

1516-8913

We analyzed the genetic relationships between 51 fungal isolates previously identified as A. niger aggregate, obtained from dried fruit samples from worldwide origin and 7 A. tubingensis obtained from Brazilian coffee beans samples. Greater fungal diversity was found in black sultanas. Aspergillus niger sensu stricto was the most prevalent species. It was found in all fruit substrates of all geographical origins. Based on Random Amplification of Polymorphic DNA (RAPD) and b-tubulin sequences data two groups of A. niger were found. In spite of the small number of isolates from Group IV an association between extrolite patterns and molecular clustering is speculated. A. tubingensis were the second most frequent species and this species were clearly subdivided into two groups. The finding of two groups for A. tubingensis strains could not yet explain the contradictions found in the literature about the capability this species for ochratoxin production, because both of them were formed by only non-ochratoxin producing strains.