Development and Evaluation of a Three-Dimensional Printer–Based DNA Extraction Method Coupled to Loop Mediated Isothermal Amplification for Point-of-Care Diagnosis of Congenital Chagas Disease in Endemic Regions

WEHRENDT, DIANA P.; ALONSO-PADILLA, JULIO; LIU, BO; ROJAS PANOZO, LIZETH; RIVERA NINA, SILVIA; PINTO, LILIAN; LOZANO, DANIEL; PICADO, ALBERT; ABRIL, MARCELO; PINAZO, MARIA J.; GASCON, JOAQUIM; TORRICO, FAUSTINO; WONG, SEASON; SCHIJMAN, ALEJANDRO G.

JOURNAL OF MOLECULAR DIAGNOSTICS

AMER SOC INVESTIGATIVE PATHOLOGY, INC

Año: 2021 vol. 23 p. 389 - 398

1525-1578

Vertical transmission of Trypanosoma cruzi is the cause of congenital Chagas disease, a re-emerging infectious disease that affects endemic and nonendemic regions alike. An early diagnosis is crucial because prompt treatment achieves a high cure rate, precluding evolution to symptomatic chronic Chagas disease. However, early diagnosis involves low-sensitive parasitologic assays, making necessary serologic confirmation after 9 months of life. With the aim of implementing early diagnostic strategies suitable for minimally equipped laboratories, a T. cruzi–loop-mediated isothermal amplification (LAMP) prototype was coupled with an automated DNA-extraction device repurposed from a three-dimensional printer (PrintrLab). The whole process takes