Oxidative and nitrosative responses of the mussel Mytilus edulis platensis during seasonality and harmful algae blooms

GONZÁLEZ PAULA M.; PUNTARULO SUSANA

Nelson

Congreso; Physiomar & ANZMBS 2021; 2021

The objectiveof the work was to study the oxidative and nitrosative metabolism of the bivalveMytilus edulis platensis after exposure to biotoxins during harmful algaeblooms (HAB) in spring. Digestive glands (DG) and hemocytes were isolated from musselsof the Argentinean Sea during summer, winter and spring. In the DG, the2′,7′dichlorofluorescein diacetate (DCFH-DA) oxidation rate, ascorbylradical/ascorbate and lipid radical (LR●)/α-tocopherol contentratios were significantly higher between samples collected during the HABseason with those of summer and winter. The antioxidant enzyme activitiesshowed different responses between seasons, and nitric oxide (NO) content was8.5- and 2.7-fold higher in samples isolated from winter and spring than valuesobtained in summer mollusks, respectively. Even more, hemocytes? DCFH-DAoxidation rate was increased by 2.5- and 8.3-fold during spring and summer,respectively, as compared to winter samples. Antioxidant enzyme activities inspring cells were significantly higher as compared to summer. LR●content in spring was 140% and 50% higher as compared to winter and summercells, respectively. NO and nitro-tyrosine content were significantly higher inhemocytes from spring as compared to values obtained in winter and summer. Thesedata suggests that under natural seasonality conditions the overall network ofoxidants/antioxidants seems to be adequately controlled to keep the organismfar from damage. However, in the presence of toxins, the bivalves were facingoxidative and nitrosative stress.