INVESTIGADORES
GONZALEZ Paula Mariela
congresos y reuniones científicas
Título:
Oxidative metabolism related to Fe content in aquatic photosyntetic and non-photosyntetic organisms
Autor/es:
GONZÁLEZ PAULA M.; MALANGA, G.; PUNTARULO S.
Lugar:
Buenos Aires
Reunión:
Encuentro; VIII Meeting of the Society Free Radical Biology and Medicine-South American Group (VIII SFRBM-SAG); 2013
Resumen:
Since
natural Fe concentrations may affect oxidative metabolism, digestive gland from
bivalves (Mya arenaria, Mytilus edulis
platensis, Mytilus edulis chilensis) and
algae (Navicula sp.), were analyzed.
Fe content in sediments of the respective analyzed species was 7.5 ± 0.6, 1.4 ± 0.4, 15 ±
1 and 33.5 ± 0.7 mg/g DW in Wadden Sea, San Antonio Oeste, Beagle Channel and
Carlini Base, respectively. Total Fe content was 0.9 ± 0.2, 0.6 ± 0.05 and 7.8
± 0.2 nmol/mg FW in M. arenaria, M. edulis plantensis and M. edulis chilensis, respectively and 18
± 2 nmol/104 cell in Navicula
sp. Labile iron pool (LIP) content showed higher values in animals with
higher total Fe content. The oxidation rate of dichlorofluorescein diacetate was
264 ± 2, 29 ± 4 and 18 ± 2 a.u./min mg prot FW in M. arenaria, M. edulis
plantensis and M. edulis chilensis,
respectively. Reactive oxygen species generation seemed controlled by ascorbate
content and catalase activity limiting lipid and ascorbyl radical content. These results showed the influence of Fe content
in the environment on the oxidative metabolism, and the presence of the LIP
could be involved in the basal stress detected.