Oxidative status of respiratory tissues of the bivalve Mya arenaria after exposure to excess dissolved iron

GONZÁLEZ PAULA M.; ABELE, D.; PUNTARULO, S.

MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2015 vol. 48 p. 103 - 116

1023-6244

The gills and mantle are the first tissues exposed to altered oxidative conditionsduring dissolved Fe uptake. The aim of this work was to characterize Feaffected oxidative metabolism on the respiratory tissues of Mya arenaria. After9 days of exposure to elevated Fe levels (500 μM of Fe as Fe-EDTA), asignificant increase in the total Fe and labile Fe pool content was found in thegills. Dichlorofluorescein diacetate oxidation rate was higher after 17 days ofexposure as compared to controls. Thiobarbituric acid reactive substancescontent increased 1.9- and 3.7-fold over controls on day 9 and 17, respectively.Both, catalase activity and nitrate and nitrite content decreased significantly ondays 9 and 17 compared with controls. Similar effects were observed inmantle, but catalase activity was not affected. The results showed that inrespiratory tissues the labile Fe pool is critically controlled to avoidradical-dependent cellular deterioration, but when the endogenous protectionmechanisms are overwhelmed, tissue injury was observed.