Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum

CABRERA, JOAQUIN; PUNTARULO, SUSANA; GONZÁLEZ, PAULA MARIELA

Life

MDPI

Lugar: Basel; Año: 2023 vol. 13

2075-1729

Domoicacid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure wasreported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA inthe marine diatom Phaeodactylum tricornutum favors reactive oxygen species(ROS) generation in the intracellular environment modifying its antioxidantcapacity. Active species production, non-enzymatic antioxidant content, andantioxidant enzyme activities over the three growth phases of P. tricornutum exposed to 64 µM DA wereevaluated. Results obtained in exponential growing cells showed atime-depending 7-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidationrate. Superoxide dismutase and catalase activities showed a 2-fold increase, andglutathione relatedenzymes activities were also significantly increased in treated diatoms ascompared to controls. However, glutathione and ascorbate contents significantlydecreased after incubation of the cells to DA. Similar effects were observed inlatent and stationary phases of cells development. These results showed that DAcould cause a severe oxidant-dependent impact on a non-toxic algae.