INVESTIGADORES
CASALI Cecilia Irene
capítulos de libros
Título:
Analysis of XBP1 Contribution to Hyperosmolarity-Induced Lipid Synthesis
Autor/es:
CASALI, CECILIA IRENE; PARRA, LEANDRO; ERJAVEC, LUCIANA CECILIA; . FERNÁNDEZ TOME, MARIA DEL CARMEN
Libro:
The Unfolded Protein Response- Methods and Protocols
Editorial:
Springer US
Referencias:
Lugar: Humana, New York, NY; Año: 2022; p. 169 - 187
Resumen:
The unfolded protein response (UPR) is a complex network of intracellular pathways that transmits signals from ER lumen and ER bilayer to the nuclear compartment in order to activate gene transcription. UPR is activated by the loss of ER capacities, known as ER stress, and occurs to restore ER properties. In this regard, glycerolipid (GL) synthesis activation contributes to ER membrane homeostasis and IRE1aplpha-XBP1, one UPR pathway, has a main role in lipogenic genes transcription. Herein, we describe the strategy and methodology used to evaluate whether IRE1alpha-XBP1 pathway regulates lipid metabolism in renal epithelial cells subjected to hyperosmolar environment. XBP1s activity was hindered by blocking IRE1alpha RNAse activity and by impeding its expression; under these conditions, we determined GL synthesis and lipogenic enzymes expression.
