Probiotic Saccharomyces cerevisiae effect on rat intestine histomorphometry after dietary aflatoxins exposure and yeast-cell ultrastructure changes induced by aflatoxin B1.

DOGI C; CRISTOFOLINI A; GONZÁLEZ PEREYRA ML.; FOCHESATO A.; MERKIS C.; DALCERO AM; CAVAGLIERI LR.

World Mycotoxin Journal

Wageningen Academic Publishers

Lugar: Wageningen; Año: 2017

1875-0710

Gastrointestinal tract (GIT) is the main site where conversion and absorption of food components takes place and the first system coming into contact with mycotoxins of dietary origin.The aim of this work was to study the effect of probiotic Saccharomyces cerevisiae RC016 on intestinal villi of rats exposed to aflatoxins (AFs) for 60-days. Moreover, the effect of in vitro AFB1 exposure on yeast cell ultrastructure, diameter and cell wall thickness was evaluated. Six treatments were applied (n=6) to inbred male Wistar rats: 1) uncontaminated feed control (F), 2) yeast control, 3) F + 40 g/Kg AFB1 + 20 g/Kg AFG1, 4) F + 100 g/Kg AFB1 + 50 g/Kg AFG1, 5) F + 40 g/Kg AFB1 + 20 g/Kg AFG1 + daily oral dose 108 viable S. cerevisiae cells, 6) F + 100 g/Kg AFB1 + 50 g/Kg AFG1 + daily oral dose 108 viable S. cerevisiae cells. Morphometric measurements (villus length and width, crypt depth, quantification of goblet cells) were assessed using image analysis. S. cerevisiae RC016 cells were exposed to 20 g/ml of AFB1 in intestinal solutions or in PBS and cells processed for transmission electron microscopy and high resolution light microscopy studies. Dietary exposure to the yeast did not induce significant differences in villus width but increased villus length and crypt depth. AFs-contaminated diets induced an increase in villus length, width and crypt depth and a significant decrease in the number of goblet cells which were improved by the addition of S. cerevisiae RC016. A significant increase in the yeast cell diameter was observed when RC016 was exposed to AFB1, suggesting this as an advantage since a larger cell would be able to adsorb mycotoxins more efficiently. The ability of this strain to act as probiotic and AFs binder makes it a candidate for the formulation of new additives to improve animal performance.