Inverse Optimal Filtering Method for the Instrumental Spreading Correction in Size Exclusion Chromatography

ALBA, D.; MEIRA, G. R.

JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHNOLOGIES

Año: 1984 vol. 7 p. 2833 - 2862

1082-6076

This paper shows the practicability of the use of the fast Fourier transform (FFT), with appropriate filtering in the frequency domain, as a means of deconvoluting Tung's integral formula (1). The method is limited to uniform instrumental spreading functions, but presents several important advantages: it is numerically efficient, no assumptions about the shape of the spreading function are made, it eliminates the highfrequency measurement noise components from the corrected chromatogram without modifying the original data, and provides a means of physically interpreting the results.