Changes in the proliferation/apoptosis balance in the bovine ovary: a key early event in follicular persistence

BELOTTI, E.M.; STASSI, A.F.; VELÁZQUEZ, M.M.; DÍAZ, P.U.; MARELLI, B.E.; REY, F.; NOTARO, U.; ORTEGA, H.H.; SALVETTI, N.R.

CELLS TISSUES ORGANS

KARGER

Lugar: Basel; Año: 2017

1422-6405

The objective of this work was to evaluate proliferation and apoptosis in the bovine ovary in a model of follicular persistence induced by low levels of progesterone, to detect incipient changes during COD development, on the expected day of ovulation (day 0) and after 5, 10 and 15 days of follicular persistence. We analyzed cell proliferation by evaluating the expression of Ki-67 and apoptosis by evaluating caspase-3, BAX and BCL2 expression. Proliferation was similar in the granulosa and theca cells of antral follicles in P0 (treated with progesterone up to the expected day of ovulation) and in the control group. A decrease in cell proliferation was detected after 5 days of persistence (P5) in relation to P0 (p