Identification and phylogenetic analysis of LysM-RLK gene family in peanut (Fabaceae).

RODRIGUEZ-MELO, JOHAN STIBEN; IBAÑEZ, FERNANDO; TONELLI, MARÍA LAURA; FABRA, ADRIANA

Buenos Aires

Congreso; The fourth International Society for Computational Biology Latin America Bioinformatics Conference (ISCB-LA).; 2016

International Society for Computational Biology

Identification and phylogenetic analysis of LysM-RLK gene family in peanut (Fabaceae).J. S. Rodríguez-Melo1, F. Ibañez1, M. L. Tonelli1 & A. Fabra1 Department of Natural Sciences. National University of Río Cuarto1LysM-RLKs (LysM Receptor-like kinases) belong to a diverse family of plant receptors involved in the recognition of elicitors like chitin from fungi and rhizobial Nod factors (NF). Perception of these signals can trigger defensive responses or activate symbiotic signaling in plants, respectively. The aim of this study was to analyze the diversity of members of LysM-RLK family in peanut, a legume whose genomic sequence has not been determined yet. By using bioinformatics approaches, we identified 31 predicted proteins from the two wild ancestral diploid genomes of cultivated tetraploid peanut, Arachis duranensis and Arachis ipaënsis, and three proteins from cultivated peanut Arachis hypogaea not previously reported. Our sequence analysis showed that all these proteins possess typical features of LysM-RLKs like three extracellular conserved LysM domains (LysM1, LysM2 & LysM3), CXC motifs in the interspacing regions and an intracellular kinase domain. Subsequently, we tried to correlate the structure and phylogeny of the receptors with their ability to perceive specific ligands. Our results showed that the ability to bind different ligands is related with substitutions in specific positions of the sequences, and not with the phylogeny or overall similarity among the receptors. Finally, similarity analyses enabled us to select three sequences putatively implicated in recognition of different molecules (chitin, NF and both). Further biological experiments and analysis by qRT-PCR will give insights into the regulation and evolution of this important gene family in peanut.Supported by: SECyT UNRC, CONICET, ANPCyT-SECyT.