PERSONAL DE APOYO
ADROVER Ezequiela
congresos y reuniones científicas
Título:
EXPRESSION OF DOPAMINERGIC TRANSCRIPTION FACTORS IN PRENATALLY STRESS RATS AT DIFFERENT AGES.
Autor/es:
M. R. KATUNAR, E. ADROVER, T. SAEZ , A. BRUSCO, AND M. C. ANTONELLI
Lugar:
Chicago, Illinois, USA
Reunión:
Congreso; 39th Anual Meeting of the Society for Neuroscience; 2009
Institución organizadora:
Society for Neuroscience (SFN)
Resumen:
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EXPRESSION OF DOPAMINERGIC TRANSCRIPTION
FACTORS IN PRENATALLY STRESS RATS AT DIFFERENT AGES.
M. R. Katunar1, E. Adrover1, , T.
Saez2, , A. Brusco2, and M. C. Antonelli1. 1IQUIFIB
(UBA-CONICET), Facultad de Farmacia y Bioquímica. UBA.
2IBCN. Facultad de Medicina.UBA. Buenos Aires. ARGENTINA.
Rats
exposed to different types of stress during the last week of pregnancy produce
offspring that show severe anomalies in neural development and brain morphology
that persist into adulthood particularly in dopaminergic neurotransmission. We have previously shown that prenatal stress (PS)
increases dopamine (DA) D2-type receptor (D2R) levels in prefrontal cortex
(PFc), nucleus accumbens (NAc) core and hippocampus (Hip) and a decrease of
amphetamine stimulated DA levels in NAc shell and PFc. It has recently been
identified two transcription factors (TFs), Nurr1 and Pitx3 which are expressed
at critical moments of DA neurons differentiation. Employing an immunocytochemistry
approach, we studied the expression levels of these TFs and of TH, the rate
limiting step enzyme in the synthesis of DA, in mesencephalon slices of
prenatally stressed rats at different ages. We found a ubiquitous distribution of
Nurr1 whereas Pitx3 remains restricted to substancia nigra (SN) and ventral
tegmental area (VTA). Our results show that the expression of both Nurr1 and
Pitx3 increased in prenatally stressed offspring in the VTA area at PND 7 and
PND 60, whereas no changes were observed in SN areas. TH expression was reduced
at PND 7 in
VTA and recovered to control values at PND 28 and 60. The increase of the
specific dopaminergic transcription factors and TH expression might be a
compensatory mechanism to counteract the reduction in dopamine levels observed in
PFC, which in turn might be upregulating D2 dopaminergic receptors. Since dopamine release is still impaired at
adult ages we speculate that other steps in the dopamine synthesis and storage
metabolism is impaired such as dopamine transporters VMAT2 or DAT. The area
selectivity of the increase of the TFs towards VTA and the mesolimbic pathway
indicates that an insult received during the prenatal period will exert mainly
motivational, emotional and reward behaviour impairments in the adult life.
