PERSONAL DE APOYO
ADROVER Ezequiela
congresos y reuniones científicas
Título:
Prenatal stress effects on morphological and neuroendocrine aspects of male rats progeny
Autor/es:
PALLARÉS M.E1.; GONZÁLEZ-CALVAR S.I.2; BOURGUIGNON N.S.3; ADROVER E1; KATUNAR M.R.1; BAIER C.J.1; LUX-LANTOS V.3;CALANDRA R.S.2; ANTONELLI M.C1
Reunión:
Congreso; VI reunion anual de la Sociedad Chilena de Neurociencias; 2010
Resumen:
We have previously demonstrated that prenatal stress (PS) exerted an impairment of dopaminergic
(DA) metabolism in limbic brain areas especially after puberty. In this study
we evaluated hypothalamic- pituitary- testicular (HPT) axis status of males exposed to PS. Stress consisted on a 3
daily- 45min restraint session from day 14th of gestation to
delivery. Anogenital distances and testis descent were measured at postnatal
day (PND) 21. Luteinizing hormone (LH),
Testosterone (T) and 5-a Androstane- 3-a, 17b- Diol (DIOL)
serum levels of 28, 35, 45, 60 and 75 days old male progeny were determined by
radioimmunoassay. Testes of 35 and 60 days old animals were processed for
histological morphometric measures. PS reduced anogenital distance of PND 21
male offspring and induced a 2-day delay in the completion of testis descent, in
comparison to controls (C). Hormones
serum levels analyses revealed that PS diminished LH at PND 28 and 75 (88 and
65,6%), decreased T serum levels at 75 days old rats (58,6%), and increased DIOL
levels at PND 28 and 45 (61 and 63%). Additionally, the rate of spermatogenesis
development was accelerated on PS rats and the mean tubular diameter was
increased. However, the mean Leydig cells number was reduced on PS rats. Our
results demonstrate that stress during gestation induces long term effects on
the male progeny HPT axis. Since it was reported that gonadal hormones produce
plastic changes in the brain, a disbalanced hormone milieu might be responsible
of the DA metabolism impairment observed in our previous studies.