SURFACE DECORATION OF VESICLES WITH GOLD NANOPARTICLES. A FAST AND RELIABLE METHOD

M. FÁTIMA BARROSO; J.S. FLOREZ-TABARES; M. A. LUNA; C. DELERUS-MATOS; N. M. CORREA; F. MOYANO; P. MOLINA

Porto

Encuentro; XX ENCONTRO LUSO-GALEGO DE QUIMICA; 2014

Sociedade Portuguesa de Química

Vesicles are small artificial spherical shape that can be created from natural non-toxic phospholipids. Due to their size and hydrophobic and hydrophilic character and biocompatibility, liposomes are promising systems for drug and gene delivery, as well as a mimic for cell membranes. In the biosensor field, vesicles find special application in signal amplification, due to their excellent carrier properties for encapsulation of signal marker compounds across a wide spectrum of sensing modalities. Additionally, the ability to modify the vesicles surface structure to perform recognition functions with a wide range of types of analytes is an important aspect of their use in bioanalysis. Moreover, the ability of Vesicle decoration with inorganic nanoparticles (NP) increases the possibilities of performs recognition functions. In this work, it was self-assembled gold nanoparticles (AuNP) on vesicles structures. In this case, the AuNP were synthetized in organic solvent, glycerol, under UV radiation. After the optimization of AuNP synthesis (irradiation time, HAuCl4 concentration), these AuNP were immobilized onto vesicles structures composed by the phospholipid DOPC (1,2-Dioleoyl-sn-glycero-3-phosphocholine). This nanosystem was characterized by using UV-VIS spectrometry, transmission electron microscopy (TEM), dynamic light scattering and cyclic voltammetry. The results show that the vesicles are decorated with gold nanoparticle of (less than 15 nm) with the advantage that the method is fast and reliable since no nanoparticle extraction is needed