Study of lipid peroxidation and ascorbic acid protective role in large unilamellar vesicles from a new electrochemical performance

BARROSO, M. FÁTIMA; LUNA, M. ALEJANDRA; MOYANO, FERNANDO; DELERUE-MATOS, CRISTINA; CORREA, N. MARIANO; MOLINA, PATRICIA G.

BIOELECTROCHEMISTRY

ELSEVIER SCIENCE SA

Año: 2018 vol. 120 p. 120 - 126

1567-5394

In this contribution an electrochemical study is described for the first time of lipid peroxidation and the role ofantioxidant on lipid protection using large unilamellar vesicles (LUVs). In order to simulate the cell membrane,LUVs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were used. A vesicle-modified electrodewas constructed by immobilizing DOPC LUVs onto carbon paste electrodes (CPEs). Lipid peroxidation was studiedelectrochemically by incubating the vesicle-modified electrodes with hydroxyl (HO?) radicals generated viathe Fenton reaction. Oxidative damage induced by HO? was verified by using square wave voltammetry (SWV)and was indirectly measured by the increase of electrochemical peak current to [Fe(CN)6]4− which was usedas the electrochemical label. Ascorbic acid (AA) was used as the antioxidant model in order to study its efficacyon free radical scavenging. The decrease of the electrochemical signal confirms the protective key role promotedby AA in the prevention of lipid peroxidation in vesicles. Through microscopy, itwas possible to observemorphologicmodification on vesicle structures after lipid peroxidation in the presence or absence of AA.