Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform

M. FÁTIMA BARROSO; M. ALEJANDRA LUNA; JUAN S. FLORES TABARES; CRISTINA DELERUE-MATOS; N. MARIANO CORREA; FERNANDO MOYANO; PATRICIA G. MOLINA

Beilstein Journal of Nanotechnology

Beilstein-Institut zur Förderung der Chemischen Wissenschaften.

Lugar: Frankfurt; Año: 2016 vol. 7 p. 655 - 663

In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim ofusing this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPsonto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine(DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored ontoSH?DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs weresuccessfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using environmentallyfriendly procedures.