Bacterial adherence on a polymeric coating applied on carbon steel

NICOLINO SANDRO; MONICA ZAPPONI; SONIA BRUNO; VERÓNICA RODRÍGUEZ BAUZADA; PABLO CASTRO; SANDRA G. GÓMEZ DE SARAVIA; MARISA R. VIERA; BLANCA M. ROSALES, SILVIA E. RASTELLI, ERNESTO G. MAFFÍA; CECILIA I. ELSNER; ALEJANDRO R. DI SARLI; MAURO BANERA

formato virtual

Congreso; 4º Congreso de Integridad y Corrosión en la Industria del Petróleo y el Gas; 2021

IAPG (Instituto Argentino del Petróleo y el Gas)

Microbial contamination in the oil and gas industry results in a variety of problems indrilling fluids, hydraulic fracturing fluids, water flooding, pipelines, and storage tanks.Microorganisms such as sulfate-reducing bacteria (SRB), acid producing bacteria, and/or other halophiles can negatively impact asset integrity and reduce the quality andquantity of produced hydrocarbons. Organic coatings are used on the internal tubesurface to prevent corrosion problems in service. The aim of this study was to evaluatethe performance of polymeric coatings applied on carbon steel in the presence of amicrobial consortium from an oil storage tank. The samples were exposed to a batchculture of the microbial community in Postgate´s C medium in anaerobic conditionsat 28 ± 2 °C for 60 days. After that time, different techniques were applied to analyzesurface deterioration: Scanning Electron Microscopy (SEM), Electrochemical ImpedanceSpectroscopy (EIS), Epifluorescence Microscopy (EM). Furthermore, the planktonic andsessile microbial communities were analyzed by Next-Generation Sequencing (NGS) of16S rRNA gene. The genetic analysis showed a shift in the bacterial community alongthe experiment. SRB were predominant at the beginning, diminishing their numberwith time. Bacteria belonging to the Desulfovibrionales and Bacterioidales orders werethe most abundant in the biofilms formed on the samples. According to the EIS results,neither deterioration of the coating, nor corrosion of the carbon steel was detectedafter the exposure to the cultures for 60 days.