Skin-Immune system dialog in an in vitro model: effects of activated lymphocytes on normal keratinocytes proliferation and cytokine production

MARIELA L. PAZ; JULIANA LEONI; DANIEL H. GONZÁLEZ MAGLIO

Córboda

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunología

Skin immune system, composed by cells and soluble molecules, can reach a state of disequilibrium originating chronic inflammatory pathologies (CIP), like psoriasis or atopic dermatitis. We aim to study the interaction of the cutaneous epithelium, particularly keratinocytes (KCs), with the immune system, particularly T helper cells. We generated a KCs' primary cell culture from C57 mice epidermis, and we polyclonally activated T cells from a C57 mice spleen with Concanavalin A. We evaluated the effects of activated T cells' secreted molecules (supernatant transference) and also the ones of Tcell-KCs contact (coculture: coc) on KCs. Cocs were performed with T cells preactivated 48 hs before or during the cocs (in situ) We measured KCs' proliferation by flow cytometry with CFSE probe and KCs' cytokine production by ELISA. KCs' cytokine concentrations were calculated subtracting the values obtained for activated T cells alone to the ones obtained in the cocs. T cells' supernatant transference produced no significant effect on KCs' proliferation or cytokine production. Cocs of KCs with preactivated T cells had no influence on KCs' proliferation but induced a significant increment in TNF-α production (p