INVESTIGADORES
FURLAND Natalia Edith
artículos
Título:
Altered Lipid Homeostasis in Sertoli Cells Stressed by Mild Hyperthermia
Autor/es:
VALLÉS, ANA SOFÍA; AVELDAÑO, MARTA ISABEL; FURLAND, NATALIA EDITH
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2014 p. 1 - 13
ISSN:
1932-6203
Resumen:
Spermatogenesis is known to be vulnerable to temperature. Exposures of rat testis to moderate hyperthermia result in loss
of germ cells with survival of Sertoli cells (SC). Because SC provide structural and metabolic support to germ cells, our aim
was to test the hypothesis that these exposures affect SC functions, thus contributing to germ cell damage. In vivo, regularly
repeated exposures (one of 15 min per day, once a day during 5 days) of rat testes to 43uC led to accumulation of neutral
lipids. This SC-specific lipid function took 1?2 weeks after the last of these exposures to be maximal. In cultured SC, similar
daily exposures for 15 min to 43uC resulted in significant increase in triacylglycerol levels and accumulation of lipid droplets.
After incubations with [3H]arachidonate, the labeling of cardiolipin decreased more than that of other lipid classes. Another
specifically mitochondrial lipid metabolic function, fatty acid oxidation, also declined. These lipid changes suggested that
temperature affects SC mitochondrial physiology, which was confirmed by significantly increased degrees of membrane
depolarization and ROS production. This concurred with reduced expression of two SC-specific proteins, transferrin, and
Wilms? Tumor 1 protein, markers of SC secretion and differentiation functions, respectively, and with an intense SC
cytoskeletal perturbation, evident by loss of microtubule network (a-tubulin) and microfilament (f-actin) organization. Albeit
temporary and potentially reversible, hyperthermia-induced SC structural and metabolic alterations may be long-lasting
and/or extensive enough to respond for the decreased survival of the germ cells they normally foster