Genomic analysis and proteomic response of the chromium‐resistant and phenanthrene‐degrading strain Streptomyces sp. MC1

SINELI, P.E.; HERRERA, H.M.; APARICIO, J.D.; GUERRERO, D.S.; POLTI, M.A.; DÁVILA COSTA, J.S.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2021

1364-5072

Aim: Chemically disparate toxic organic and/or inorganic molecules producedby anthropogenic activities often hinder the bioremediation process. Thisresearch was conducted to understand the capacity of Streptomyces sp. MC1 toremove chemically disparate toxics such as Cr(VI) or phenanthrene.Methods and Results: Genomic, metabolic modeling and proteomicapproaches were used in this study. Our results demonstrated that Streptomycessp. MC1 has the genetic determinants to remove Cr(VI) or degradephenanthrene. Proteomics showed that these genetic determinants wereexpressed. Metabolic versatility of the strain was confirmed by two metabolicmodels in complex and minimal media. Interestingly, our results also suggesteda connection between the degradation of phenanthrene and synthesis ofspecialized metabolites.Conclusions: Streptomyces sp. MC1 has the genetic and physiological potentialto remove Cr(VI) or degrade phenanthreneSignificance and Impact of Study: The probability of a microorganism tosurvive in the presence of different contaminants depends on its geneticpotential and the ability to express it. The genetic and proteomic profilesobtained for Streptomyces sp. MC1 can be recommended as model and predictif other Streptomyces strains can be used in bioremediation processes. Ourwork also hypothesized that intermediates of the phenanthrene degradationserve as precursors for the specialized metabolism.