Effects of the cytokines on proliferation of hippocampal adult neural progenitor cells.

ALEJANDRO DAVID MORONI; ANA MARÍA GENARO; MARÍA LAURA PALUMBO

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias 2017; 2017

SAIC, SAIB, SAI, SAA, SAB, SAB, SAFE, SAFIS y SAH

The hippocampus, an area involved in learning and memory, is sensitive to the effects of chronic stress. Cytokines have been shown to affect some behaviour, including memory. Moreover, IL-1b, IFN-g and IL-6 had been implicated in psychiatric disorders. Previously, we found that chronic stress induced a cognitive deficit that was correlated with a decrease in adult neurogenesis and Th1/Th2 imbalance. Recently, we observed a decrease in IFN-g mRNA levels in hippocampus and lymph nodes and increase IL-4 expression in lymph nodes in stressed mice. On the other hand, adult hippocampal neurogenesis is initiated with the proliferation of neural progenitor cells (NPCs) within the dentate gyrus leading to the formation of new neurons. Regulation of this process occurs via numerous secreted factors such as cytokine. The aim of this work was to study the effect of IFN-g, IL-1b, IL-4 and IL-6 on the NPCs proliferation. NPCs were obtained from hippocampus of adult BALB/c mice. NPCs were culture in vitro in optimal conditions with growth factors. In the last 72 h, each cytokine (0.1, 1 and 50 ng/ml) were added and BrdU 2 h before to fix the culture. The proliferation was measure by immunocytochemistry using DAPI and anti-BrdU-Cy3. We found that cytokine concentration of 50 ng/ml inhibit the proliferation of the NPCs respect to control without cytokine: IFN- (95%, p0.01), IL-1b (100%, p0.001), IL-4 (41%, ns) and IL-6 (43%, ns). Concentrations of 1 ng/ml of IFN-g (85%, p0.01) and IL-1b (77%, p0.01) also reduced the proliferation of the NPCs. No significant differences were found in culture treated with IFN-g (0.1ng/ml) and IL-4 and IL-6 (1 and 0,1 ng/ml) respect to control. These results indicated that high concentration of cytokines inhibit the proliferation of hippocampal NPCs. Low concentrations of cytokine no affect the NPCs proliferation. These findings are the first in this field. More studies are necessary to determinate the direct role of cytokine on adult NPCs.