Comparative sequence analysis and adipose-specific expression of G0S2 and ATGL in sheep

AHN J; WU H; SUH Y; CARRANZA A; RELLING A; SHIN S; LEE S; LEE K

JOURNAL OF SMALL RUMINANT RESEARCH

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017

0921-4488

Lipolysis is a catabolic reaction that is mediated by adipose triglyceride lipase (ATGL) and its inhibitor such as G0/G1 switch gene 2 (G0S2) protein during the first step of hydrolysis. The hydrophobic domain of G0S2 protein interacts and binds to the patatin domain in ATGL. Although conservation of the hydrophobic domain has been reported across species, sheep G0S2 amino acids and its hydrophobic domain have not been sequenced and studied. In this study, the hydrophobic region of sheep G0S2 protein was highly conserved suggesting its role in inhibition of ATGL-mediated lipolysis in sheep. Phylogenetic analysis revealed that ruminant G0S2 proteins showed greater homologies within the ruminant species compared to other mammalian species. Moreover, adipose-specific expression of G0S2 for sheep was observed. Together with an abundant expression of ATGL protein in adipose tissue of sheep, G0S2 protein may play a critical role in inhibiting ATGL-mediated triacylglycerol (TAG) hydrolysis in the adipose tissue