Morphometrical comparative analysis between alcoholic and diabetic parotid sialosis

BOHL LUCIANA; MERLO CAROLINA; CARDA CARMEN; GÓMEZ DE FERRARIS MARÍA ELSA; CARRANZA MIRIAM

Madrid

Congreso; XIII Congreso Nacional de la Sociedad Española de Histología e Ingeniería Tisular. I International Congress of Histology and Tissue Engineering; 2005

Sociedad Española de Histología e Ingeniería Tisular

n previous studies, we observed that alcoholic and diabetic parotid sialosis shows morphological characteristics that indicate the existence of a differential qualitative histopathological feature. In order to compare the morphometrical modifications among both pathologies, in parotid biopsies of patient with sialosis of diabetic and alcoholic etiology, we measured different variables in acini and ducts and relative quantity of fatty tissue in stroma. Samples of 5 biopsies of parotid of patient with diabetes mellitus type 11 and 12 of patient with alcoholic hepatic cirrhosis were used. Both groups with clinical signs of glandular swelling. Seven samples of patient without these antecedents were used as control. Samples were embedded in paraffin and stained with routine techniques. Morphometrical study was carried out using a digital image analyzer (Optimas ©) attached to a video camera and an optic microscope (Axiophot Zeiss) with a magnification power of 400. Direct records were obtained in acini and ducts and with these data indirect parameters were calculated. In a randomized microscopic field (40X) we calculated the relative quantity of fatty tissue. AII data were processed using the "U" test of Mann Whitney (p