Biochemical and cellular shifts in the blood of Pomacea canaliculata induced to different hypometabolic states

RODRIGUEZ, C; CRUZ, C; GIRAUD BILLOUD, M

Mendoza

Congreso; XL Reunión Anual de la Sociedad de Biología de Cuyo; 2022

Sociedad de Biología de Cuyo

Exposure to cold (hibernation) and desiccation (estivation) induces hypometabolic states in Pomacea canaliculata (Caenogastropoda, Ampullariidae), where different adaptive defense mechanisms allow them to tolerate it, like the preparation for oxidative stress that protects tissues during the arousal of the quiescence. Although the growing body of knowledge of this adaptive strategy, little is known about what happens in the internal environment of the animal and the hemolymph of snails exposed to hypometabolic situations, and the potential alterations in the immunological system. In this study, animals were exposed to short cycles of activity-quiescence-arousal, where quiescence consisted of 7 days hibernating animals, keeping in aquariums at 11ºC or estivating them by keeping the animals out of the water for 7 days. The arousal consisted of put the animals in water under active animal conditions (control, water at 24-26 ºC) for 30 minutes, to then obtain the samples. Once the condition of each experimental group was met, hemolymph samples were obtained and used to determine the concentration of hemocyanin, uric acid, total cell count, and also to make a morphological characterization of the different cell types by means of flow cytometry. The cycle of activity-estivation-arousal (AEA) induced a significant increase in the concentration of hemocyanin in arousal, which was maintained in this way in the arousal group, compared to the control (ANOVA-Dunnett, P