Evaluation of female masculinization in Pomacea canaliculata (Caenogastropoda, Ampullariidae) induced by tributyltin, heavy metals, and uranium in culture water

GIRAUD BILLOUD, M; CAMPOY DÍAZ, AD; MANSILLA, E; VEGA, ISRAEL A.

Environmental Health and Toxicology

Korean Society of Environmental Health and Toxicology & Korea Society for Environmental Analysis

Año: 2022

2233-6567

All female Pomacea canaliculata develop a small, male-like copulatory apparatus a few days after birth, which growths slowly until sexual maturity, and grows even further in older age. Previous studies have found trace elements like mercury (Hg), arsenic (As) and uranium (U) in tap water used for snail culture, and these elements were accumulated in snail tissues. Here, we test whether the presence of these metals at maximum allowed concentrations (Environmental protection agency - EPA) in aquarium water could affect the development of the copulatory apparatus in mature female. Females of different ages were used as controls, grown in reconstituted metal-free water with or without the addition of mercury, arsenic and uranium, as well as tributyltin, a compound used as masculinizing agent. Six and seven months old females cultured in tap water showed a longer penis and penile sheath, and a greater overall development of the copulatory apparatus, measured by an index (DI), as compared with same-age females cultured in reconstituted water. Moreover, when females were exposed to mercury, arsenic or uranium at the maximum contaminant levels (MCLs) for human consumption allowed by EPA regulations, there was no further development of the copulatory apparatus, while there was a clearly positive effect in TBT-exposed females. This study confirms the masculinizing effect of organotin compounds on female copulatory apparatus and discusses the usefulness of the development of these organs as a bioindicator of environmental pollution.