Phenolic metabolites in plasma and tissues of rats fed with a grape pomace extract as assessed by liquid chromatography-tandem mass spectrometry

RODRIGUEZ LANZI MC; PERDICARO DJ; ANTONIOLLI A; PICCOLI P; VAZQUEZ PRIETO MA; FONTANA AF

Mendoza

Simposio; Simposio Internacional de Medicina Traslacional; 2019

Facultad de Ciencias Médicas, Universidad Nacional de Cuyo

Grape pomace extract (GPE) is a rich and relatively low-cost source of phenolic compounds. However, little is known about the main GPE metabolites in mammals, which could help explain the observed health-promoting effects. This study investigated the presence of parent compounds from flavanol, flavonol and stilbene families and their metabolites in rat plasma and tissues after an acute intake of GPE in doses of 300 and 600mgkg/body weight. The measurement of free compounds and their metabolites was performed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Results showed the presence of epicatechin, epicatechin methyl-glucuronide, epicatechin methyl-sulphate, catechin, catechin-glucuronide, quercetin methyl-glucuronide, resveratrol-3-glucuronide, resveratrol-4-glucuronide and resveratrol-3-sulphate in plasma, which was dose dependent. The most abundant measured compound in plasma was epicatechin-glucuronide. The presence of glucuronidated and methyl-glucuronidated forms of catechin were observed in the liver at both doses, while epicatechin-glucuronide andmethyl-glucuronide were detected onlyuponintake of600mg GPE/kg body weight. At this dose epicatechin-glucuronide and methyl-glucuronide were also detected in muscle, and catechin methyl-glucuronide inadipose tissue.Results showthe mainGPEmetabolites presentinrattissuesafter oral consumption, contributing to better understand the health benefits of GPE and its potential utilization as a functional ingredient.