Grape pomace extract induced mitochondrial biogenesis in visceral adipose tissue from spontaneously hypertensive rats receiving a high-fat diet and in primary rat adipocytes

RODRIGUEZ LANZI MC; PERDICARO DJ; ANTONIOLLI A; FONTANA AF; BOTTINI R; MIATELLO RM; VAZQUEZ PRIETO MA

Davis, California

Congreso; Oxidants and Antioxidants in Biology: Redox Medicine and Nutrition; 2016

Oxygen Club of California

We determinate the capacity of malbec grape pomace extract (GPE), containing high amount of polyphenols, to modulate inflammation and proteins related with mitochondrial biogenesis in epididymal visceral adipose tissue (eVAT) from spontaneously hypertensive rats (SHR) receiving a high-fat diet (HFD) and in primary cultures of rat adipocytes. Ten weeks old SHR (n=16) and its control WKY (Wistar-Kyoto) rats (n=16) were divided into three groups: Control group (WKY/SHR; n=4 each); WKY/ SHR-HFD group receiving fat (40% (w/w), n=6 each); and WKY/SHR HFD supplemented with GPE (300 mg/Kg/d, n=6 each) for 10 weeks. HFD leads to increased eVAT in WKY but not in SHR, while the adipocyte diameter was higher in both groups. GPE supplementation decreased the adipocytes size, NOX4 and IL-6 and enhances UCP-1 and PPARɣ expression in SHR-HFD. To evaluate the involvement of p38 and ERK MAPKs on UCP-1 activation, the stromal vascular fraction from eVAT were isolated from WKY and SHR and differentiated to adipocytes in presence or absence of SB203580 (p38 inhibitor) or U0126 (ERK inhibitor) (10 mmol/L) and GPE (30 M). We first confirm that GPE enhance UCP-1 and PPARγ expression in adipocytes from SHR but not from WKY and that p38 and ERK were involved in GPE-induced UCP-1 expression in SHR adipocytes. GPE can stimulates the expression of protein involved in mitochondrial biogenesis and may protect against diet-induced adipose dysfunction supporting the utilization of winemaking residues in human health due to their high amount of bioactive compounds.