NADPH-oxidase (NOX) and nitric oxide synthase (NOS) in high blood pressure: modulation by dietary interventions

GALLEANO M; PIOTRKOWSKI B; CALABRÓ V; LITTERIO MC; PRINCE PD; COSTA MA; VAZQUEZ PRIETO MA; OTEIZA PI; FRAGA CG

Kyoto

Congreso; 17 th Biennial Meeting of Society for Free Radical Research International; 2014

Background: NOX and NOS activities are key players to determine NO steady-state concentration, being their modulation relevant factors in the control of BP and other specif organ functions. Methods: NOX and NOS were studied in aorta, heart and kidney of rats with increased BP by: a)L-NAME (360mg/l in beverage, 4d); and b) fructose (10% in beverage, 8wk). In both models, the effect of dietary intervention with (-)-epicatechin (EC) was analyzed. Results: In L-NAME rats, the three tissues showed increased NOX activity (2-3 times higher than control rats). As opposite, L-NAME treated rats showed a strong decrease in NOS activity (61% of decrease respect to the control values). EC (360mg/kg bw, 4d) avoided hypertension development in association with restoration of NOS and NOX activities to control values. In fructose-overload rats, with a moderate increase in BP, the three tissues showed both NOX and NOS activities significantly increased. EC (20 mg/kg bw, 8wk) prevented the increase in BP, in association with restoration of NOX activity to normal values. Interestingly, NOS activity was found increased in the three tissues to values significantly higher than the corresponding to fructose-overload rats. Conclusion: Increase in NO steady-state concentration would be a central mechanism for EC action.