Quercetin induced white adipose tissue browning and attenuate high-fat diet-induced glucose intolerance: implications of the FNDC5/irisin pathway in muscle and in L6 myotubes.

PERDICARO DJ; RODRIGUEZ LANZI C; MUSCIA V; OTEIZA PI; VAZQUEZ PRIETO MA

Congreso; Asociación Latinoamericana de Ciencias Fisiológicas (ALACF); 2020

ALACF y Sociedad Chilena de Ciencias Fisiológicas

Introduction: Irisin is an exercise-induced myokine that can induce browning of white adipose tissue (WAT), through upregulation of the uncoupling protein-1 (UCP-1), and other metabolic benefits. We previously observed that supplementation with the flavonoid quercetin (Que) mitigated high-fat diet (HFD)-induced glucose intolerance and adipose hypertrophy. Aim: To evaluate whether these beneficial effects could be related to Que capacity to activate muscle FNDC5/irisin and UCP-1 and brown markers in the subcutaneous WAT (SAT) of rats consuming a HFD. Also, the role of Que on FNDC5/irisin pathway in L6 myotubes triggered with palmitate. Methods: Rats (n=21) were divided in 3 groups: Control (Ctrl), HFD, and HFD supplemented with Que (20 mg/kg body weight/d) for 6-weeks, (protocol approval N° 36/2014). L6 myotubes were treated with/without Que (1uM) and subsequently with/without palmitate (0.5 mM). Statistical analysis: Data are shown as mean ± S.E.M. Statistical significances were assessed by one-way ANOVA followed by Bonferroni?s Test. A P