SUPPLEMENTATION WITH GRAPE POMACE EXTRACT, RICH IN PHENOLIC COMPOUNDS, MITIGATES HIGH FAT DIET-INDUCED LIVER STEATOSIS IN MICE

MUSCIA SAEZ, MV; PERDICARO DJ; ASENSIO J; GARCÍA R; COSTANTINO V; VAZQUEZ PRIETO MA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2022

SAFIS, SAIC, SAI

Obesity is one of the major public health concerns worldwide. Obesity can increase the risk to develop insulin resistance, cardiovascular disease, dyslipidemia and non-alcoholic fatty liver disease among others. Grape pomace (GP) is a waste product, containing predominantly a left-over of skins and seeds, generated in the winemaking process which contains relatively high amounts of bioactive components like polyphenols. The aim of this study is to evaluate the effect of grape pomace extract (GPE), containing high amount of phenolic compounds, on the consequences and the metabolic complications associated with high fat diet (HDF)-induced obesity in mice. 8- week-old C57BL/6 male mice (20-25g) were divided into 4 different groups (n=7 each one) and fed for 14 weeks as follow: i) control group: standard diet, ii) control with GPE group: control diet supplemented with GPE 300mg/kg body weight/day, iii) high-fat diet group (HFD): control diet containing 60% of the total calories from fat (lard), iiii) HFD supplemented group: high fat diet supplemented with GPE 300mg/kg body weight/day. Consumption of a HFD significantly increased body, adipose tissue and liver weight. In addition, the HFD increased plasma cholesterol, decreased glucose tolerance as evidence by glucose tolerance test and increased liver fat infiltration. GPE supplementation decreased these variables and consequently prevented fat infiltration into hepatocytes in mice fed with HFD. Overall, these findings highlight the utilization of GPE rich in bioactive compounds in the attenuation of liver steatosis and obesity-related alterations.