INVESTIGADORES
LO NOSTRO Fabiana Laura
artículos
Título:
Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)
Autor/es:
MUÑOZ-PEÑUELA M.; LO NOSTRO, F.; DAL´ OLIO GOMES A.; TOLUSSI, C.E.; SOUZA BRANCO G; SILVA PINHEIRO, J. P.; ANDRADE DE GODOI F.; GUIMARAES MOREIRA, R.
Revista:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. TOXICOLOGY & PHARMACOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2021 vol. 240 p. 1 - 8
ISSN:
1532-0456
Resumen:
The concentration of pharmaceutical compounds in the aquatic ecosystems are low, nevertheless can cause toxic effects to the organisms. The aim of this study was to evaluate the effects of diclofenac (DCF), a non-steroidal anti-inflammatory, and caffeine (CAF), a central nervous system stimulant, alone or combined, in Astyanax altiparanae males under an acute exposure (96 h), measuring biomarkers of neurotoxicity, antioxidant response and damage at molecular and cellular levels. DCF concentration in the water, separated and combined, was 3.08 mg L-1 and for CAF 9.59 mg L-1. To assess neurotoxicity, brain and muscle acetylcholinesterase (AChE) activities were measured. To evaluate oxidative stress, the enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), as well as lipoperoxidation (LPO), were analyzed in liver and gills. The activity of hepatic cyclooxygenase (COX) was also evaluated. Genotoxicity was assessed in blood using Comet assay and micronucleus test, as well as nuclear abnormalities. DCF and CAF, alone or combined, had neither effect on AChE activity, nor in the activity of SOD, CAT, GPx and GST. In liver DCF inhibited the activity of SOD and GPx; CAF inhibited the activity of CAT. The mixture inhibited the activity of SOD and GST, although only animals exposed to CAF increased hepatic LPO. Under these experimental conditions, no effect on COX activity was observed, as well as no cytotoxic and genotoxic damage. DCF and CAF altered the activity of hepatic antioxidant enzymes in A. altiparanae males; only CAF triggers hepatic LPO.
