INVESTIGADORES
LO NOSTRO Fabiana Laura
artículos
Título:
Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using sGnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae).
Autor/es:
M. RAVAGLIA; F. LO NOSTRO; M. C. MAGGESE; G. GUERRERO; G. SOMOZA
Revista:
FISH PHYSIOLOGY AND BIOCHEMISTRY
Editorial:
Kluwer Academic
Referencias:
Lugar: Holanda; Año: 1997 vol. 5 p. 425 - 436
ISSN:
0920-1742
Resumen:
This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain
of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon
GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final maturation
and gamete release in this species. Evidence for the presence of two GnRH variants, sGnRH and
cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay
with different antisera. The effects of treatment with sGnRH-A+DOM were checked by
three ways: oocyte and milt release by stripping, histological analysis of the gonadal tissue, and androgen
serum levels at different times throughout the experiment. In males, spermiation was induced after three
weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation at the end of the
experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal.
All the treated fish had elevated androgen serum levels from the third week, with respect to control fish.
In all cases, serum estradiol levels were undetectable. These results suggest that treatment with sGnRH
analog and DOM induce sex reversal in female and spermiation in males.Synbranchus marmoratus, and the effects of the administration of salmon
GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final maturation
and gamete release in this species. Evidence for the presence of two GnRH variants, sGnRH and
cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay
with different antisera. The effects of treatment with sGnRH-A+DOM were checked by
three ways: oocyte and milt release by stripping, histological analysis of the gonadal tissue, and androgen
serum levels at different times throughout the experiment. In males, spermiation was induced after three
weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation at the end of the
experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal.
All the treated fish had elevated androgen serum levels from the third week, with respect to control fish.
In all cases, serum estradiol levels were undetectable. These results suggest that treatment with sGnRH
analog and DOM induce sex reversal in female and spermiation in males.