Brucella suis ΔmapB OUTER MEMBRANE VESICLES POTENTIAL AS VACCINE CANDIDATES AGAINST BRUCELOSIS.

FLORENCIA MUÑOZ GONZALEZ; IVÁN ALONSO PAIVA; BIALER, MAGALÍ G.; BALDI PABLO C; FERRERO MARIANA CRISTINA

Congreso; LXIX Reunión de la Sociedad Argentina de Inmunología; 2021

Outer membrane vesicles (OMVs) have been used in development of vaccines against Gramnegative bacteria. Here we evaluated B. suis M1330 (wt) and B. suis ∆mapB OMVsimmunogenicity and protective capacity against both systemic and mucosal B. suis challenge.Female Balb/c mice were immunized intramuscularly (i.m.) with OMVwt (20 μg), OMVΔmapB(20 μg) or saline at 0 and 30 days. One week after last immunization serum, bronchoalveolarlavage, feces and saliva samples were obtained to measure OMV-specific antibodies. One-week later mice were challenged with virulent B. suis through the intraperitoneal (i.p.) orintratracheal (i.t.) routes. CFU counts were determined in lungs and/or spleens 20 days afterchallenge. Antibodies capacity to neutralize Brucella infection was determined in lung epithelialcells culture (A549 cell line).Vaccination with both OMVs induced serum specific IgG (p