Brucella infection of human endometrial stromal cells induces a proinflammatory response

FERRERO MARIANA CRISTINA; ANDREA GISELLE FERNÁNDEZ; FOCARACCIO JULIETA; IVÁN ALONSO PAIVA; MUÑOZ GONZALEZ F; SOTELO AGUSTINA; CANELLADA ANDREA; BALDI PABLO C

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; 2018

Sociedad Argentina de Inmunología

Brucella infection of human endometrial stromal cells induces a proinflammatory responseFerrero MC, Fernández AG, Focaraccio J, Alonso Paiva IM, Muñoz González F, Sotelo A, Canellada A, Baldi PCBrucellosis can induce abortion in humans and animals. Therefore, the interaction between uterine cells and Brucella is important for understanding the pathogenesis of this disease. Inflammatory phenomena have been shown to promote abortion by other pathogens. In this work, we evaluated the inflammatory response related to Brucella infection of a human endometrial stromal cell line (T-HESC). For this, T-HESC cells were decidualized in-vitro, infected with B. abortus 2308 or B. melitensis H38, or stimulated with conditioned medium (CM) from infected human macrophages, and changes in cytokines and chemokines were measured by ELISA. We demonstrate for the first time that B. abortus and B. melitensis can infect and proliferate in T-HESC. Expression levels of the decidualization marker prolactin did not change in Brucella-infected T-HESC as compared to non-infected T-HESC (182±13 pg/ml for B. abortus, 176±14 pg/ml for B. melitensis vs. 192± 8 for non-infected cells). The infection induced an increase in interleukin8 (IL-8: 3233± 112 pg/ml for B. abortus, 3907±140 pg/ for ml B. melitensis vs. 1154± 188 pg/ml for non- infected), IL-6 (18 ±2 pg/ml, 17 ±2.3 pg/ml vs 0± 0 respectively) and monocyte chemoattractant protein 1 (MCP-1: 4022±298 pg/ml, 2253±42 pg/ml vs. 1375 ±106 pg/ml respectively) secretion at 48 h post- infection. Further, the stimulation of T-HESC for 48 h with CM from B. abortus-infected macrophages induced a significant increase of IL-8 and MCP-1 as compared to stimulation with CM from non-infected macrophages (p