INTRANASAL IMMUNIZATION WITH THE NOVEL BtaF TRIMERIC AUTOTRANSPORTER FROM Brucella abortus IN A MURINE MODEL.

FLORENCIA MUÑOZ GONZALEZ; SYCZ GABRIELA; PAIVA ALONSO IVAN; FÉRNANDEZ GISELLE ANDREA; FOCARACCIO JULIETA; BALDI, PABLO CÉSAR; ZORREGUIETA ANGELES; FERRERO MARIANA CRISTINA

Buenos Aires

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2017

Brucella spp. can be transmitted to humans through mucosae. Currently, there are no human vaccines against this infection. We have previously shown that BtaF is an adhesin involved in the attachment of Brucella spp. to biotic and abiotic surfaces, and that it is important for the dissemination and persistence of the pathogen after intra-traqueal murine infection. Based on these results, we evaluated BtaF as a potential component of an acellular vaccine against brucellosis.Six-week-old Balb/c mice were weekly immunized for 3 weeks with BtaF (10ug) plus 3?,5?-c-di-AMP (c-di-AMP,10ug), c-di-AMP alone or saline. One week after last immunization serum, saliva, feces, bronchoalveolar (BAL) and vaginal lavage (VL) fluids, lung homogenates and spleens were obtained. Levels of BtaF-specific antibodies were measured by indirect ELISA in all samples. In vitro production of gamma interferon (IFN-γ), interleukin-5 (IL-5) and IL-2 by spleen cells stimulated with BtaF (10 µg), ConA or RPMI was determined. Immunized mice showed increased serum levels of total antibodies against BtaF (p