PROINFLAMMATORY RESPONSE OF HUMAN ENDOMETRIAL EPITHELIAL CELLS TO Brucella abortus INFECTION

FERNANDEZ ANDREA GISELLE; FERRERO, MARIANA CRISTINA; RITTER ANDREA; FLORENCIA MUÑOZ GONZALEZ; IVAN ALONSO PAIVA; FOSSATI, CARLOS ALBERTO; PABLO C BALDI

Buenos Aires

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2017

Sociedad Argentina de Inmunología

Brucella induces abortion in humans and animals. Therefore, theinteraction between uterine cells and Brucella is important for understandingthe pathogenesis of this disease. In this work, we evaluatethe inflammatory response (chemokine and metalloproteinases)and the expression of hormonal receptors and various microRNArelated to infection or inflammation processes in the human endometrialepithelial cell line Ishikawa, after infection with B. abortus2308 or stimulation with conditioned medium (CM) from infected humanphagocytes or trophoblasts. The Ishikawa cells, the monocyticcell line THP-1, the trophoblastic cell line Swan-71 and monocytesand neutrophils isolated from peripheral blood were infected with B.abortus for 2 h. After treatment with gentamicin, supernatants werecollected at 24 and/or 48 h. Here we show that B. abortus invadesand replicates in Ishikawa cells without inducing an increase in interleukin8 (IL-8) or monocyte chemoattractant protein 1 (MCP-1)secretion. However, the stimulation of Ishikawa cells for 48 h withCM from B. abortus-infected THP-1 cells, primary monocytes orSwan-71 cells induces a significant increase of IL-8 and MCP-1 ascompared to stimulation with CM from non-infected cells (p