INVESTIGADORES
FERRERO Mariana Cristina
congresos y reuniones científicas
Título:
CCL20 IS SECRETED BY LUNG EPITHELIAL CELLS IN RESPONSE TO BRUCELLA ABORTUS INFECTION AND HAS ANTIMICROBIAL ACTIVITY AGAINST THIS PATHOGEN
Autor/es:
FERRERO MC; HIELPOS, MS; FERNÁNDEZ, AG; FOSSATI CA; BALDI PC
Lugar:
Los Cocos, Córdoba
Reunión:
Congreso; LXI Reuníon Anual de la Sociedad Argentina de Inmunología; 2013
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Título: CCL20 IS SECRETED BY LUNG
EPITHELIAL CELLS IN RESPONSE TO BRUCELLA ABORTUS INFECTION AND HAS
ANTIMICROBIAL ACTIVITY AGAINST THIS PATHOGEN.Autores : MARIANA CRISTINA FERRERO ; María Soledad Hielpos
; Andrea Giselle Fernandez ; Carlos Alberto Fossati ; Pablo César Baldi
IDEHU
Resumen
:
Although Brucella
frequently infects humans through inhalation, its interaction with pulmonary
cells has been overlooked. CCL20 is a potent chemoattractant for immature
dendritic cells and T cells. Antimicrobial activities for CCL20 have been
recently reported. We evaluated the antimicrobial activity of CCL20 against Brucella
abortus, and whether this pathogen induces the secretion of CCL20 in human
bronchial (Calu-6) and alveolar (A549) epithelial cells and macrophages
(PMA-treated THP-1 cells). CCL20 killed B. abortus with a lethal dose
(LD50, achieving 50% reduction of CFU) of 50 ug/ml. Cells were infected for 2
hours at multiplicities of infection (MOI) of 50 to 500 bacteria/cell
(epithelial cells) or 10 to 100 bacteria/cell (macrophages). At 24 and 48 h
post-infection CCL20 was measured by ELISA in culture supernatants. Infection
significantly increased the production of CCL20 in macrophages and bronchial
epithelial cells in a MOI-dependent manner (p<0.05). Both cell types also
secreted CCL20 in response to heat-killed B. abortus and to PAMPs used
for comparison (flagellin, Pam3Cys and E. coli LPS). In contrast, B.
abortus LPS did not induce CCL20 secretion. Whereas the infection of A549
alveolar cells induced a slight, non-significant increase of CCL20 (p>0.05),
the stimulation of these cells with conditioned medium from B. abortus-infected
macrophages (CoIM) induced CCL20 secretion in a dose dependent manner
(p<0.05). Neutralization assays with the antagonist of IL-1β receptor (IL-1Ra) showed that IL-1β present in CoIM is mostly
responsible for this induction, whereas an anti-TNFα antibody had no effect. This study shows that
bronchial epithelial cells and macrophages secrete CCL20 upon infection with B.
abortus or stimulation with its antigens, while alveolar epithelial cells
do so after stimulation by factors secreted by infected macrophages. CCL20
has antimicrobial activity against B. abortus.