INVESTIGADORES
FERRERO Mariana Cristina
congresos y reuniones científicas
Título:
Chemokine and matrix metalloproteinase response of Swan-71 trophoblastic cells to infection with Brucella abortus and to factors produced by Brucella-infected monocytes
Autor/es:
FERNÁNDEZ, AG; FERRERO, MC; HIELPOS, MS; DELPINO MV; FOSSATI CA; BALDI PC
Lugar:
Los Cocos, Córdoba
Reunión:
Congreso; LXI Reuníon Anual de la Sociedad Argentina de Inmunología; 2013
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Brucella is well known to produce
abortion in humans and animals, presumably by inducing a proinflammatory
response that affects fetus viability. In this work we evaluated the cytokine
and matrix metalloproteinase (MMP) response of human trophoblasts to Brucella infection. Swan-71, a human trophoblastic
cell line, was infected with B. abortus
at multiplicities of infection (MOI) from 50 to 500. After treatment with
gentamicin, cells were lysed at different times post-infection (p.i.) and
lysates were plated onto bacteriological agar. For each time point, intracellular
CFU increased with increasing MOI. In addition, CFU counts increased between 2
and 24 h p.i., indicating the capacity of Brucella
to replicate in trophoblasts. This finding was confirmed by confocal microscopy
using DsRed-labeled Brucella. A
significant increase of TNF-α (p<0.05) levels was detected in supernatants
from infected cells (MOI 100 and 500), whereas IL-8, MCP-1 and IL-10 did not
increase. MMP-9 activity as detected by zymography was unchanged except for a
decrease at MOI 500 (p<0.05). To verify the capacity of trophoblasts to respond
to PAMPs, Swan-71 were stimulated with different TLRs agonists. An increase of
IL-8 was detected after stimulation with Pam3Cys and flagellin (p<0.01),
with no change in IL-10 and TNF-α. No response to E. coli LPS was detected. To
evaluate the interaction of trophoblasts and monocytes during infection (a
feasible situation in the maternal-fetus interface), Swan-71 were stimulated
with supernatants from Brucella-infected
THP-1 cells (a monocytic cell line), detecting an increase in IL-8, MCP-1
(p<0.001) and MMP-9 (p<0.05) but no change in IL-10 and TNF-α. These
findings suggest that Swan-71 cells produce TNF-α upon Brucella infection and secrete chemokines and MMP-9 upon interaction
with Brucella-infected monocytes. The
resulting inflammatory environment, together with modifications in MMPs levels,
may affect embryo implantation and lead to abortion.