IL-1R and Inflammasomes Mediate Early Pulmonary Protective Mechanisms in Respiratory Brucella abortus Infection

HIELPOS MARÍA SOLEDAD; ANDREA GISELLE FERNÁNDEZ; FALIVENE, JULIANA; PAIVA ALONSO IVAN; FLORENCIA MUÑOZ GONZALEZ; FERRERO, MARIANA CRISTINA; CAMPOS PRISCILA C; COSTA OLIVEIRA SERGIO; BALDI PABLO C; VIEIRAS NAGELICA T

Frontiers in Cellular and Infection Microbiology

Fontiers Media S.A.

Lugar: Laussane; Año: 2018 vol. 8 p. 1 - 9

2049-9957

Brucella spp. infection is frequently acquired through contaminated aerosols. The role of interleukin-1 beta (IL-1b) in the early pulmonary response to respiratory Brucella infection is unknown. As shown here, IL-1b levels in lung homogenates and bronchoalveolar lavage fluid (BALF) of mice intratracheally inoculated with B. abortus were increased at 3 and 7 days p.i. At 7 days p.i., pulmonary CFU numbers were higher in IL-1 receptor (IL-1R) knockout (KO) mice than in wild type (WT) mice. At different times p.i. CFU in lungs and BALF were higher in mice lacking some inflammasome components (caspase-1, AIM2, NLRP3) than in WT mice. At 2 days p.i. pulmonary levels of IL-1b and CXCL1 (neutrophils chemoattractant) were lower in caspase-1/11 KO mice. At day 3 p.i., neutrophils counts in BALF were lower in caspase-1/11 KO mice than in WT mice. During in vitro infections, IL-1b secretion was lower in alveolar macrophages fromcaspase-1/11, NLRP3 or AIM2 KO mice than in WT controls. Similarly, IL-1b production by B. abortus-infected alveolar epithelial cells was reduced by pretreatment with a specific caspase-1 inhibitor. This study shows that IL-1R, probably through IL-1b action, and the NLRP3 and AIM2 inflammasomes are involved in pulmonary innate immune protective mechanisms against respiratory B. abortus infection.