Endoplasmic Reticulum Stress and Autophagy Contribute to the Functional Remodeling of the Anterior Pituitary Gland in Diet-Induced Insulin Resistance

MERCAU, MARÍA ELISA; REPETTO, ESTEBAN M.; PEREZ, MATÍAS; MARTINEZ CALEJMAN, CAMILA; SANCHEZ, ROCÍO; OLEJARCZYK, JUAN M.; FINKIELSTEIN, CARLA; CYMERYNG, CORA BEATRIZ

Chicago

Congreso; 16th INTERNATIONAL CONGRESS OF ENDOCRINOLOGY AND 96TH ANNUAL MEETING AND EXPO OF THE ENDOCRINE SOCIETY; 2014

THE ENDOCRINE SOCIETY

              The goal of this study was to evaluate the alterations caused by a sucrose rich diet (SRD) on the hypothalamic-pituitary-adrenal (HPA) axis of male Wistar rats, and the therapeutic effect of a moderate exercise (E) protocol in animals with an insulin-resistant (IR) phenotype. The protocol was implemented at different stages of the treatment, in order to analyze whether the effect of the SRD could be prevented and/or reversed by E. Rats were randomized into 6 groups (n=5): Control and SRD-fed sedentary rats (C-S, SRD-S), C and SRD-fed rats that exercised daily since week 1 (C-E, SRD-E), C and SRD-fed animals that exercised daily since IR was confirmed (C-R, SRD-R). The treatments were carried out for 15 weeks. On week 7, Insulin Tolerance Test (ITT) was performed to confirm the IR state of SRD fed animals. Statistical significance was analyzed by ANOVA followed by Tukey´s post hoc test.First, we analyzed the effect of the SRD in the sedentary group. Serum metabolites such as fasting glucose (p