METOCLOPRAMIDE – NITRITE REACTION. VALIDATION OF ITS APPLICATION TO THE SPECTROPHOTOMETRIC ANALYSIS OF GENERIC TABLETS

DELFINO, MARIO RAUL (H); MONZON, CELINA MARÍA; HERNANDEZ, SILVIA R,; SARNO, MARÍA DEL CARMEN

IOSR Journal of Pharmacy

International Organization of Scientific Research

Año: 2012 vol. 2 p. 27 - 34

2250-3013

A quantitative method based on the product of the reaction between the antiemetic metoclopramide and sodium nitrite in acidic medium by visible spectrophotometry is proposed. Cooling or coupling stage are unnecessary, due to the stability of the colored diazo compound, which has a maximum absorbance at 380 nm. This procedure was applied to pharmaceutical formulations containing metoclopramide as single drug. Analytical validation of the proposed method compared to the reference method, HPLC – UV was performed. The parameters of specificity, precision (CV = 0.994% and 1.58% for repeatability and intermediate precision respectively), accuracy (recovery between 98.50 to 101.33%), linearity and linear range obtained, fall within the values established by the British Pharmacopoeia, USP and Argentinian pharmacopoeia.