Production of enzyme cocktails by fungal consortiums

MORILLA, ESTEBAN AMADOR; WOITOVICH VALETTI, NADIA; PELLEGRINA MALPIEDI, LUCIANA; TUBIO, GISELA

Congreso; Biocatalysis Open Day; 2020

The production of enzymes that constitute enzyme cocktails is carried out by fungalfermentations consisting of an association of different strains coexisting in a singlefermentation step. These mixed fermentations are industrially and economicallypotential because the costs and production times of enzyme cocktails can beconsiderably reduced.The aim of this work was to evaluate the feasibility of using a fungal consortium byAspergillus niger and oryzae, and Trichoderma harzanium for the production of anenzyme cocktail enriched in xylanolytic (XE), cellulolytic (CE), amylolytic (AE),proteolytic (PE) and lipidic (LE) enzymes using agro-industrial waste like wheat bran,soybean husk and sunflower husk as substrates. Then, the strains were evaluated tocoexist in the same culture by interaction studies and the production of antagonisticmetabolites. Different fungal consortium models were tested in selected agroindustrial waste.Solid-state (SSF) and submerged (SmF) fermentation were assayed for 5 days at 30°C inall agro-industrial wastes. The activity of XE, CE and AE were measured by thedinitrosalicylic acid colorimetric method, PE activity was measured by the azocaseinmethod and LE by the colorimetric method based on the hydrolysis of pNPB.The combination of wheat bran and soybean husk proved to be the most favorablesource of C and N for maximum enzyme production after 4 days of fermentation at30°C for A.niger and oryzae. These species demonstrated to be able to coexist in afungal consortium because no mycelium could occupy the territory occupied by theother microorganism. A. niger and A. oryzae formed a fungal consortium thatproduced IU/gds of substrate of XE (65), CE (30), AE (135), PE (13,000) and LE (1.95).The results obtained suggest that the fungal consortia consisting of A. niger and A.oryzae was an efficient design for the production of hydrolytic enzyme cocktails.