Shotgun proteomics to study Bordetella pertussis adaptation to stressful conditions

LAMBERTI Y; SCHMIDT, FRANK; SURMANN, KRISTIN; CAFIERO, HILARIO; RODRIGUEZ ME

Congreso; LXII Reunión Científica Anual de la Sociedad Argentina de Inmunología (SAI); 2014

Previous studies indicated that B. pertussis (Bp), the etiologic agent of whooping cough, is able to survive inside human macrophages. The events involved in the establishment of intracellular infection are not fully understood, but nutrient limitation and reduced oxygen tension are believed to be factors that Bp has to face during infection. The present study was undertaken to provide insight into Bp protein expression during these stressful conditions. We used LC-MS/MS to compare the proteomes of Bp growing under oxygen-sufficient and oxygen-limited conditions, and under nutrient starvation. A total of 1290 proteins of Bp were identified. More than 140 proteins changed significantly their expression under low-oxygen conditions, whereas 200 proteins show an altered expression during nutrient starvation (p