The impact of BP1092 on Bordetella pertussis virulence factor regulation

MARTINA DEBANDI; MARIELA CARRICA; CHRISTIAN HENTSCHKER; UWE VÖLKER; MARIA EUGENIA RODRIGUEZ; KRISITIN SURMANN; YANINA LAMBERTI

Simposio; 13th International Bordetella Symposium; 2022

Virulence factors play a key role in Bordetella pertussis (Bp) persistence inside host cells.Transcription of Bp virulence factors is controlled by the interrelated two-component systems(TCS) BvgAS and RisAK and potential further, yet unknown, regulatory systems. BP1092 is aTCS histidine kinase with increased protein levels upon internalization by a human macrophagecell line (Lamberti et al., JProteomics-2016).Here, we further characterized the regulatory contribution of BP1092 under virulent and avirulentconditions by global proteomics. To this end, we performed mass spectrometric analysis of BpTohama I wildtype (wt), an isogenic BP1092 deficient mutant (ΔBP1092), and ΔBP1092trans-complemented with BP1092 (ΔBP1092 pBBR-BP1092) under control (SS) and modulating(SS + 40 mM MgSO4) conditions.We found ten proteins with altered abundance between ΔBP1092 and wt or thetrans-complemented strain, respectively, under modulating conditions but none under controlconditions. Among the proteins with different abundance (fold change >1.5, q1.5, q>0.05, p